Pgps3 Unique Restriction Sites Here

: To perform an in vitro transposition reaction, the donor DNA (transposon) must often be linearized or confirmed. Unique sites like XbaI and HindIII are frequently used to digest the pZero backbone or the pGPS3 itself to release the genomic insert for efficient recombination.

: For directional cloning, researchers often use pairs like BamHI and NotI , which are closely spaced (733 and 741 bp) but have distinct overhangs. pgps3 unique restriction sites

If you want to replace the 35S promoter with a tissue-specific promoter (e.g., RBCS or AtUBQ10 ): : To perform an in vitro transposition reaction,

: The Transprimer-1 element in pGPS3 contains a kanamycin resistance ( KnRcap K n to the cap R-th power If you want to replace the 35S promoter

For precise mapping, you can refer to the official pGPS3 Sequence Map provided by . pGPS3 - 4293 bp - NEB